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. 2017 Sep 12;7:11333. doi: 10.1038/s41598-017-11882-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The roles of OsHsp18.0 in thermo- and salt-tolerance. (a) OsHsp18.0-overexpressing lines OE-3 and OE-6 exhibited higher thermotolerance than wild-type Nipponbare. The 4-d seedlings of Nipponbare and transgenic lines were subjected to heat treatment. The representative plants were photographed after recovery at 28 °C for 14 d. Bars = 2 cm. (b) The thermotolerance of OsHsp18.0-silenced transgenic plants SE-18 and SE-12 was reduced compared with wild-type SH5 plants. (c) Comparisons of the thermotolerance indexes between transgenic and wild-type plants. The thermotolerance indexes were calculated as described in Materials and Methods. The asterisks indicate that a significant difference (*p < 0.05; **p < 0.01) was detected between transgenic plants and the wild type. (d) OsHsp18.0-overexpressing transgenic lines OE-3 (column 1) and OE-6 (column 2–3) displayed enhanced tolerance to salt stress relative to the wild-type Nipponbare. (e) OsHsp18.0–silencing lines SE-18 and SE-12 were more sensitive to 200 mM NaCl than wild-type SH5. The photos in (d) and (e) were taken at 5 d post salt treatment.