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. 2017 Sep 12;7:11333. doi: 10.1038/s41598-017-11882-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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OsHsp18.0 is localized in both cytoplasm and nuclear envelope. Agrobacteria solutions carrying GFP-OsHsp18.0 and free RED constructs driven by the 35S promoter were infiltrated into N. benthamiana leaves, respectively. At 2 d post infiltration, the infiltrated leaf areas were cut and the fluorescence images were captured by confocal laser scanning microscopy. The white bar represents 25 μm (top row) or 7.5 μm (bottom row).