Fig. 5.

Immunogold electron microscopy of intact flagellar filaments from C. haemolyticum. a Flagellar filaments (control lacking antibody). b Flagellar filaments labeled with gold 10-nm nanoparticle-conjugated affinity purified polyclonal antibodies raised against the C. haemolyticum flagellin FliA(H)-hypervariable region metallopeptidase domain. Gold nanoparticles can be seen decorating the surface of individual flagellar filaments c and flagellar bundles d, revealing that the metallopeptidase domains are surface-exposed and distributed evenly throughout filaments. Scale bars are 100 nm in length