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. 2017 Sep 12;7:11385. doi: 10.1038/s41598-017-09829-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) The 3D schematic of the 3D-µdialysis chip, including the enlarged image of the openmili chamber which is the staining region dwelling on the top of PDMS porous membrane with an array of holes, and the cross section view illustrating the micro dialysis channel beneath the porous membrane. (b) Assembly of a 3D µdialysis chip, including layer A: PDMS chip containing mili chamber and micro dialysis channel, layer B: PDMS porous membrane, and layer C: Glass substrate. (c) and (d) are the SEM images of the PBS solution inlet for micro dialysis channel and the entrance to the bottom of the mili chamber region (beneath the porous membrane). (e) and (f) are the SEM images of the PDMS porous membrane with an array of holes (7 μm holes with 20 μm space).