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. 2017 Sep 12;7:11361. doi: 10.1038/s41598-017-11625-y

Figure 3.

Figure 3

Effect of PB2-701 and 702 mutations on viral growth kinetics in mammalian and avian cells. Mammalian MDCK cells were infected by wild-type and mutant viruses at an MOI of 0.01 at (a) 33 °C, (b) 37 °C, and (c) 39 °C. Avian DF-1 cells were infected by wild-type and mutant viruses at an MOI of 0.1 at (d) 33 °C, (e) 37 °C, and (f) 39 °C. Supernatant was harvested at 12 h, 24 h, 48 h and 72 h post-infection. The viral titre was determined by plaque assay in MDCK cells. Data was presented as mean ± 1 s.d. (n = 3).