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. 2017 Sep 12;7:11248. doi: 10.1038/s41598-017-11294-x

Figure 2.

Figure 2

Cumulative seed germination of six Caragana species under different temperature- and water treatments. Seeds of species from arid (C. korshinskii, ●), semiarid (C. intermedia, ○, and C. microphylla, ■), semihumid (C. arborescens, □, and C. boisi, △), and humid (C. stipitata, ▽) habitats incubated at alternating (12/12 h) temperatures of 20/10 °C (a), 25/15 °C (b), 30/20 °C (c), and at water/osmotic potentials of PEG-6000 solution of 0.0 MPa (d), −0.2 MPa (e), −0.4 MPa (f), −0.6 MPa (g), −0.8 MPa (h), −1.0 MPa (i), −1.2 MPa (j), −1.6 MPa (k) and −1.8 MPa (l) at 20/10 °C. No seeds of C. boisi and C. stipitata absorbed water and germinated at any temperature and water/osmotic potential, and no seeds germinated in any species at a water/osmotic potential of −2.0 MPa (data not shown). Data are means ± one SE of the mean of the final germination percentage when larger than the symbol (n = 5); the lines are the fitted logistic curve (see Methods).