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. 2017 Sep 12;7:11338. doi: 10.1038/s41598-017-11092-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Imidacloprid Resistance in Cyp6g1KO Backgrounds. The effect of the removal of Cyp6g1 from 3 backgrounds on imidacloprid response is shown. Control lines are shown as dark colours while lighter colours represent knockouts. Removing Cyp6g1 from RAL_517 increased imidacloprid susceptibility at both (A) 100 ppm and (B) 25 ppm. No significant changes were observed when Cyp6g1 was removed from either Canton-S or Wxac using a discriminatory dose of 5 ppm. Error bars represent the standard error of the mean. Stars represent the significance of the difference between the two genotypes measured by the Students T-test corrected for multiple comparisons with the Bonferroni method (*p < 0.05; **p < 0.001; ***p < 0.0001).