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. 2017 Sep 12;7:11338. doi: 10.1038/s41598-017-11092-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The Overexpression of Cyp6g1 and Cyp6g2 Confers Imidacloprid Resistance. The HR_GAL4 driver was used to overexpress Cyp6g1 (magenta) and Cyp6g2 (orange) from a common insertion site and their imidacloprid resistance was compared to their background control (grey). The Wiggle Index bioassay measured imidacloprid resistance and was performed at (A) 20 ppm and (B) 40 ppm in order to assess the magnitude of each gene’s ability to confer resistance. Error bars reflect the standard error of the mean. Significant differences were detected between all genotypes at all doses (ANOVA Tukey’s honestly significant difference) with p-values reported in Supplementary Table S6.