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. 2017 Sep 12;7:11282. doi: 10.1038/s41598-017-11717-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Parasporal inclusions of chimeric Cyt1Aa-BinA synthesized using the 4Q7 acrystalliferous strain of Bacillus thuringiensis subsp. israelensis. (A) Schematic of the cyt1Aa-binA gene fusion. A 0.84-kbp fragment containing the cyt1Aa gene BtIII promoter (PBtIII) and cyt1Aa open reading frame (ORF) was cloned in frame with a 1.4 kbp fragment harboring the binA ORF flanked by its native transcription terminator (ter). The nucleotide sequences at the fusion site (underlined) and the coded amino acids (KLGA, lysine-leucine-glycine-alanine) are shown above the HindIII site, as are the positions of applicable restriction sites and the 20-kDa like chaperone-like protein gene under control of the cry1Ac gene promoter (Pcry1Ac) used for cloning in pBU4 to generate the expression vector pBU-cyt1Aa-binA. The Cyt1Aa-BinA protoxin is composed of 623 amino acids and has a molecular mass of 69.6 kDa; the predicted proteolytically active forms of Cyt1Aa (22.7 kDa) and BinA (38.8 kDa) are shown. (B) Micrograph (x1000) of 4Q7/pBU-cyt1Aa-binA grown for 48 hr showing sporulated cells with endospores (s) and parasporal inclusions (c); free spores and inclusions are also present, which is typical after lysis of B. thuringiensis cells.