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. 2017 Sep 12;7:11351. doi: 10.1038/s41598-017-11829-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HIF-2α activation affects expression of various genes in cultured primary tubular epithelial cells (TECs). (A) After isolation of TECs from transgenic mice, doxycycline (DOX) was added to the media or not (control), and HIF-2α induction in TECs by DOX was confirmed by western blotting and immunofluorescent staining (n = 3). (B) In RT-qPCR for the mRNA (n = 3). qPCRs were performed 3 to 4 times with RNA isolated lysates derived from 3 independent cell culture experiments. The cropped blots are displayed; full-length blots are presented in Supplementary Fig. 5. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. controls; ^# P < 0.05, ^## P < 0.01, and ^### P < 0.001 between the two groups; bar graphs show mean ± SEM. Scale bars, 20 μm.