Figure 5.

α-GalCer treatment decreases the percent of TCRγδ⁺IL-17⁺ cells and reduces IL-17 production in mesenteric lymph nodes. Wild type BALB/c mice pretreated with 20 mg of streptomycin received 3–4 × 10³ CFU of Salmonella. Samples were taken at day 1 post-infection. Flow cytometry. The number of TCRγδ⁺IL-17⁺ cells was identified by CD3⁺TCRγδ⁺ double positive cells and gated by intracellular staining of IL-17⁺ (left panel). Uninfected mice, Control group (A); infected mice, EC group (B); infected mice treated with α-GalCer, GC EC group (C); uninfected mice treated with α-GalCer, GC group (D). Percentage of TCRγδ⁺IL-17⁺ cells (solid bars) and IL-17 expression in mesenteric lymph nodes (stripped bars) (E). Expression was determined by qPCR, levels for mRNA were determined and are presented in relation to 18S rRNA. Results are expressed as mean ± SD (n = 4). ^*Significant differences in the percentage of TCRγδ⁺IL-17⁺ cells with respect to the other three groups (p < 0.05); NS, no significant differences. ^¥Significant differences in IL-17 expression with respect to the other three groups (p < 0.05). Representative data from three independent experiments.