Figure 4.

AnkX co-localizes with Rab35 and MHC-I, a Rab35-dependent cargo. (A) Confocal images of HeLa cells co-transfected with plasmids encoding mCherry and GFP-Rab35 or plasmids encoding mCherry-AnkX and GFP-Rab35. Insets show magnified view of an area of interest. Bottom panel insets highlight an area where fluorescent signal from the two proteins co-occurred on intracellular vacuolar compartments. (B) HeLa cells ectopically producing mCherry or mCherry-AnkX were allowed to internalize monoclonal fluorescently labeled MHC-I antibodies for 30 min, followed by an acid wash. Bottom panel insets highlight the compartments where mCherry-AnkX co-occurred with internalized MHC-I.