Table 2.
The differentiation and fold-changes of known conserved sRNA expression levels under different growth conditions based on the M. huakuii 7653R RNA-seq data.
| Conserved sRNA | Candidate | Length | Sequence start and end (transcriptional direction) | FC(rpkm) | MN(rpkm) | SN(rpkm) | MN/FC | SN/FC |
|---|---|---|---|---|---|---|---|---|
| ctRNA (pMhu7653Ra) | MH_s1 | 62 | 2599–2660(−) | 1, 394.33 | 381.45 | 365.20 | 0.27 | 0.26 |
| MH_s1* | 126 | 2602–2727(+) | 101.16 | 50.98 | 60.11 | 0.50 | 0.59 | |
| ctRNA (pMhu7653Rb) | MH_s4 | 98 | 2418–2515(−) | 911.45 | 302.92 | 314.35 | 0.33 | 0.34 |
| MH_s4* | 131 | 2344–2474(+) | 147.02 | 157.55 | 104.17 | 1.07 | 0.71 | |
| tmRNA | MH_s19 | 90 | 3053513–3053602(−) | 163.27 | 72.76 | 70.51 | 0.45 | 0.43 |
| RNase | MH_s20 | 403 | 3136500–3136902(−) | 692.90 | 1, 039.98 | 1, 205.31 | 1.50 | 1.74 |
| 6S RNA | MH_s32 | 140 | 5092499–5092638(−) | 1, 244.63 | 1, 253.26 | 1, 299.15 | 1.01 | 1.04 |
| 4.5S RNA | MH_s40 | 289 | 6301885–6302173(+) | 523.54 | 883.04 | 859.06 | 1.69 | 1.64 |
*
Additional transcription was detected in the opposite strand.
FC represents free-living cells, MN represents mature nodules (28 dpi) and SN represents senescent (50 dpi) nodules. No means the gene expression in free-living cells is zero.