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. 2017 Sep 8;11:503. doi: 10.3389/fnins.2017.00503

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Copyright © 2017 Carvalho, Turunen, Wassmer, Luna-Velez, Xiao, Bennett and Vandenberghe.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Schematic representation of dual AAV strategies with size of each left and right construct. (A) Oversized monocistronic Rainbow construct showing order of reporter genes separated by furin-2A peptide cleavage sites. (B) Normal sized control construct (CMV.lacZ). (C) Overlap (OL) dual AAV strategy showing split left and right constructs and reconstituted genome structure. (D) Hybrid (HB) dual AAV strategy (top) and the equivalent reconstituted genome structures underneath. (E) Trans-splicing (TS) dual AAV strategy (top) and overview of the possible concatameric permutations for TS vectors with the highlighted combination (green box) that would lead to a correctly reconstituted genome. OL-C, HB-C, and TS-C denominate the structure of the control plasmid used in Figure 2A.