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. 2017 Sep 8;11:503. doi: 10.3389/fnins.2017.00503

Figure 2.

Figure 2

In vitro validation of dual AAV strategies. (A) Comparison of expression levels of β-galactosidade from plasmid transfection of different iterations of reconstituted Rainbow constructs (OL-C, HB-C, and TS-C) and CMV.lacZ coding control plasmid (LacZ). Two tailed Student's t-test was used to determine significance compared to CMV.lacZ (*p < 0.01). Error bars represent S.E.M. (B) In vitro efficiency comparison of AAV2 dual hybrid (HB), trans-splice (TS) and overlap (OL) to monocistronic CMV.lacZ (LacZ) construct of β-galactosidase protein expression. (C) Dose experiment comparison of AAV2 HB dual constructs at three different doses (MOI of 1E7, 1E8, and 1E9) with each dose relative to CMV.lacZ at the equivalent dose while (D) shows expression levels of the three doses of HB relative to CMV.lacZ control at the 1E7 dose. In vitro data are represented as mean ± S.D. from 1 to 2 assays done in triplicates and values are shown as percentage of control (CMV.lacZ) after background subtraction (L construct only) and corrected for plasmid reconstitution rates (using data from graph A).