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Intracellular localization of hantavirus in Caco-2 cells. Confocal laser-scanning microscopy of infected cell monolayers. Caco-2 cells growing on coverslips were infected with Puumala virus at MOI of 0.1. The cells were fixed with methanol-free formaldehyde and stained with antibodies against PUUV nucleocapsid protein (red) and early endosomal antigen 1 (EEA1, green). Cell nuclei were stained by DAPI (blue).
