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. 2017 Sep 8;8:1721. doi: 10.3389/fmicb.2017.01721

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Copyright © 2017 Witkowski, Perley, Brocato, Hooper, Jürgensen, Schulzke, Krüger and Bücker.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Intragastral infection of hamster by Puumala virus. Syrian hamsters (animal identification numbers given on the x-axis) were infected with either 1,000 plaque forming units (PFU), 10,000 PFU or 10,000 PFU γ-irradiated PUUV. Thirty five days post infection hamsters were bled to test for seroconversion by N-ELISA. Dots represent hamsters for which subsequent Andes virus challenge was lethal. Specific OD sum is the sum of the optical densities (ODs) greater than background, and represents the area under the titer curve. (B) Survival curves for hamsters “vaccinated” intragastrically with Puumala virus. Forty two days post intragastric Puumala virus challenge, the same hamsters were challenged with 200 PFU ANDV i.m.