Fig. 4.
Changes in LP spiking responses to CCAP application as measured by f-I analysis. Data represented as means ± SE. Spike frequencies were measured in all 5 experimental groups (n = 5 per group) during 5 different current injection amplitudes (from 1 nA to 5 nA, duration = 2 s) in the absence of CCAP (NO CCAP) as well as in 4 different concentrations of CCAP. Data are represented as both raw spike frequency (top) and Δ spike frequency from the “NO CCAP” state (bottom). Results of 2-way ANOVA are shown. Post hoc comparisons among experimental groups are denoted as in Fig. 3 (groups labeled with different superscripted letters show significant differences from one another). Because no interaction effects were detected, we report the post hoc results for the group factor only. Representative recordings of an LP cell from each experimental group are shown at bottom left. These traces were generated from a 3-nA current injection for 2 s in 10−7 M CCAP.