Fig. 4.

Glioma-derived IFN-α or IFN-β induces autocrine signaling. (A) IFN-α or IFN-β mRNA expression levels were determined in LN-308, LN-229, T-325, T-269, ZH-161, ZH-305, or S-24 cells using real-time PCR (median expression levels ± SE are shown from 2 independent experiments). (B, C) SiRNA-mediated gene silencing of IFN-α (si_IFN-α) or IFN-β (si_IFN-β) was performed using electroporation in T-325 or ZH-161 cells. MxA mRNA expression was determined 24 h posttransfection by real-time PCR (median expression levels ± SE are shown from 2 independent experiments) (B), while pSTAT1, STAT1, and MxA protein levels were assessed at 48 h following transfection by immunoblot. Actin was used as a loading control (1 out of 3 independent experiments is shown) (C).