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. 2017 May 16;19(10):1350–1360. doi: 10.1093/neuonc/nox061

Fig. 4.

Fig. 4

LDOC1 gene expression is silenced through DNA methylation. (A) LDOC1 gene expression measured by qRT-PCR after 7 days of 5AZA-DC treatment. Cells were treated with 10 μM 5AZA-DC refreshed every 3 days. Experiments were completed in triplicate and * denotes P < 0.05. (B) Immunofluorescence staining of LDOC1 (green) after 7 days of 10 μM 5AZA-DC refreshed every 3 days. Experiments were completed in triplicate and images are representative. (C) Quantification of LDOC1 expression after 5AZA-DC treatment ratio of nuclear LDOC1 to DAPI signal.