Fig. 5.

5AZA-DC treatment led to decreased RELA transcription and suppression of NF-κB activity. (A) Treatment with 10 μM 5AZA-DC of EPN cell lines for 7 days with refreshing every 3 days. RELA gene transcription by qRT-PCR normalized to untreated (UT) control. (B) IL-6 secretion measured by ELISA (right) in pg/mL. Experiments were repeated in triplicate and * denoted P < 0.05. (C) Quantification of RELA expression after 5AZA-DC treatment ratio of nuclear RELA to DAPI signal. (D) Immunofluorescence staining of RELA after 10 μM 5AZA-DC treatment; 100 ng/mL TNF-α stimulation for 1 h. RELA staining in green and nucleus stained with DAPI (blue).