Fig. 6.

LDOC1 regulates RELA transcription and activation of NF-κB signaling in GpA EPN. (A) IL-6 secretion measured from ex vivo patient-derived organotype slice cultures. Red indicates patients with low LDOC1 gene expression and blue denotes patients with high LDOC1. Replicate points denote cultures when material allowed for more than 1 well to be plated. (B) LDOC1 knockdown by lentiviral delivery of shRNA targeting LDOC1. Knockdown was validated by qRT-PCR. (C) IL-6 secretion by ELISA from cells after LDOC1 knockdown. (D) RELA gene expression after LDOC1 knockdown by qRT-PCR, which was normalized to shNT. Experiments were completed in triplicate and * denotes P < 0.05. (E) NF-κB target genes upregulated following LDOC1 knockdown. Experiments were performed in triplicate. Venn diagram displays number of genes upregulated with each construct compared with shNT.