FIG 4 .

Clustering of mutants based on shared transcriptional patterns of expression used to perform de novo motif discovery resulting in a motif. (A) Hierarchically clustered heat map showing the between-strain differential expression (log₂-fold change) of all transcripts that were significantly different in at least one mutant background under nitrogen-limited and balanced conditions. Clear clusters have been differentiated to allow for motif discovery analysis. (B) Enlargement of the transcriptional pattern in cluster A. (C) Discovered motif from cluster A aligned with the FixK motif.