TABLE 1 .
Bacterial strains, plasmids, and primers used in this study
| Strain, plasmid, or primer |
Relevant characteristic(s) or sequence | Reference |
|---|---|---|
| S. meliloti | ||
| Rm1021 | SU47 str-21 Smr, wild type | 91 |
| Rm11134 | Rm1021 acsA2::Tn5 Smr Nmr | 92 |
| Rm11347 | Rm1021 phbB::ΩSpSm Smr Spr | 93 |
| Rm11401 | Rm1021 ΔbdhA (in-frame deletion 1-3) Smr | 58 |
| SmUW41 | Rm1021 ΔphbA Smr | This work |
| SmUW234 | Rm1021 ΔphbAB Smr | This work |
| SmUW235 | Rm1021 ΔphbC Smr | This work |
| SmUW236 | Rm1021 ΔphaZ Smr | This work |
| Rm11479 | Rm1021 glgA1Δ PstI Smr | 15 |
| E. coli | ||
| DH5α | F−
endA1 glnv44 thi-1 recA1 relA1 gyrA96 deoR nupG purB20 φ80dlacZΔM15 Δ(lacZYA-argF)U169 hsdR17(rK− mK+) λ− |
94 |
| MT616 | MT607/pRK600, mobilizer | 95 |
| Plasmids | ||
| pK19mobsacB | Suicide vector, sacB+, mobRK2, oriR6K, Nm-Kmr | 96 |
| pRK600 | Mobilizer plasmid for conjugal transfer, Cmr Nm-Kms | 95 |
| pUC57 | Harbors synthesized constructs | |
| pMA188 | pGEMTEasy carrying phbA deletion crossover PCR product | 62 |
| pMA190 | pK19mobsacB carrying phbA deletion crossover PCR product subcloned from pMA188 |
62 |
| pGEMTEasy | Promega | |
| Primers | ||
| SMc03879A | CGCGAATTCATCAACTGGCGGAGAAAG | |
| SMc03879B | TGCGAGGATCAGGTCGTGGTGGGGACGTTCCTCATATTTG | |
| SMc03879C | CACCACGACCTGATCCTCGCAGAGTGGGAGGCGAGTATG | |
| SMc03879D | GCTCTAGAGCCGGAAAGCCGAGGGCAC | |
| SMc03879F | CGCAAGCTTCATGAGCAATCCCTCGATC | |
| SMc03879R | GCTCTAGATTACAGGCGTTCCACGCAC |