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. 2017 May 15;36(36):5199–5211. doi: 10.1038/onc.2017.4

Figure 2.

Figure 2

VEGFA increases motility and invasion via slug induction in breast cancer cells. (a and b) SNAI1, SNAI2, ZEB1 and ZEB2 expression levels were assayed by QPCR at indicated times after 10 ng/ml VEGFA in MDA-MB-231 (a) and in MCF12A (b). (c–e) The MDA-MB-231 cells were transduced with siSNAI2 or control siRNA (C) for 48 h. The cells were then treated ±10 ng/ml VEGFA for 7 days and then recovered for assays of SNAI2 and Slug expression by QPCR and western, respectively (c) and assays of migration (d) and matrigel invasion (e). (f and g) The SUM149PT cells were transduced with siSNAI2 or control siRNA for 48 h then treated ±10 ng/ml VEGFA for 7 days followed by assays of SNAI2 and Slug expression by QPCR and western, respectively (f) and by real-time matrigel invasion assay (g). All graphs show mean±s.e.m. Mean values were compared by Student's t test. * denotes P<0.05 for test versus control.