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. 2016 Apr 11;14(9):770–782. doi: 10.1038/cmi.2016.14

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Copyright © 2016 Chinese Society of Immunology and The University of Science and Technology

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Expression of homing receptors on Tregs in the spleen. Sensitized mice were EPIT-, OIT-, SLIT- or sham-treated for 8 weeks. At the end of treatment, the spleen cells were harvested for flow cytometry analysis. Using the same gating strategies, the cells were gated on CD4+CD25+Foxp3+ and the percentages of expression of homing receptors were analyzed, as shown by a representative histogram and dot plot (a). The percentages of cells expressing CCR4 (b), CCR6 (c), CXCR3 (d), CCR3 (e), CCR8 (f), CLA (g) or CCR9 (h) were analyzed. Double staining was used for the expression of CLA and CCR9 to analyze the percentage of CD4+CD25+Foxp3+ cells expressing either only CLA (CCR9-CLA+) (i), both CCR9 and CLA (CCR9+CLA+) (j) or only CCR9 (CCR9+CLA−) (k). The data are shown as the median with range from three independent experiments with n=6 for each group of mice. *P<0.05; **P<0.01; ***P<0.001; ^#P<0.05 compared with EPIT.