Fig. 2.

Exercise training reduced HHcy-induced SIRT1 inhibition. (A) The protein levels of SIRT1, p-AMPK and p-PKC in the aortic endothelium of the control group, the 1% methionine group, and the 1% methionine plus exercise group were investigated by Western blot analysis. (B, C, D) The bars represent the relative protein levels of SIRT1, p-AMPK and p-PKC, which were normalized to β-actin, and indicate the mean ± SD (n = 8 in each group). *P < 0.05, significant differences compared to the control group. #P < 0.05, significant differences compared to the 1% methionine group.