FIG 6 .

rgpA and tagO are involved in rhamnan synthesis in L. lactis MG1363. (A to C) Quantitative levels and composition of total CWPS (containing rhamnan and the PSP) in wild-type L. lactis NZ9000 and rgpA and tagO conditional mutants. (A) Total amount of CWPS. (B) Amount of each monosaccharide. (C) Rha/Gal molar ratio. rgpA and tagO conditional mutants were grown without nisin (−) or with nisin at 0.1 ng/ml (+). CWPS were extracted from cell walls with HF for 48 h at 4°C. Monosaccharides were quantified by HPAEC-PAD after TFA hydrolysis. Mean values were calculated from two independent cultures, each analyzed three times. (D) TEM of L. lactis rgpA and tagO conditional mutants grown without (−) or with (+) nisin.