FIG 2 .

NADase supports SLO protein abundance. (A and B) The abundance of SLO was evaluated by Western blotting (A) and hemolytic activity (B) of culture supernatants of Δnga, nga(stop), and nga(G330D) GAS strains; Table 1 shows strain phenotypes. (C) Transcript abundance of slo for GAS strain 854 derivatives. Data are normalized to the wild-type (wt) strain and represent the mean ± standard deviation from three experiments. The significance of differences between groups was evaluated by one-way analysis of variance with Tukey’s posttest (*, P < 0.05; **, P < 0.01; ***, P < 0.001).