Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Sep 12;8(5):e01382-17. doi: 10.1128/mBio.01382-17

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017 Velarde et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

PMC Copyright notice

FIG 7 — The C-terminal catalytic domain (190NADase) but not the N-terminal translocation domain (NT194NADase) binds independently to SLO as determined by analytical gel filtration. (A) Analytical gel filtration on a Superdex 200 Increase 10/300 GL column revealed a shift in the elution volume for the catalytic domain of NADase (190NADase) in the presence of SLO. SDS-PAGE and Coomassie blue staining of the peak fraction showed bands corresponding to both SLO and 190NADase. (B) A similar shift in elution volume was not seen for the translocation domain (NT194NADase) in the presence of SLO.