TABLE 1 .
GAS strains used in this study
| Strain | Genotype/properties | Reference |
|---|---|---|
| 854 | Wild-type M1 invasive isolate | 42 |
| 854 Δslo | slo deletion strain | 3 |
| 854 Δnga | nga deletion strain | 3 |
| 854 nga(stop) | Stop codon at amino acid 29 of NADase | This study |
| 854 nga(G330D) | Asp substitution at Gly330 of NADase, catalytically inactive | 3 |
| 854 slo(Y255A) | Ala substitution at Tyr255 of SLO, inhibits pore formation but allows for NADase translocation | 3 |
| 854 slo(Y255A)/nga(G330D) | Double mutant for inactive NADase and pore-deficient SLO | 3 |