Table 2. Morphological analysis of blood cell types in the kidney marrow from SOX4 transgenic fish compared with wild type.
Total number of zebrafisha (n=65) | Myeloid progenitors (blasts/blast-like; %) | Myelomonocyte/neutrophil (%) | Lymphocyte (%) | Immature erythroid (%) | Mature erythroid (%) | M/E ratio |
---|---|---|---|---|---|---|
5M-wild-type (n=5) | 5.27±0.74 | 45.00±5.36 | 15.33±1.36 | 10.27±1.82 | 24.13±3.19 | 5.08±1.57 |
9M-wild-type (n=5) | 5.73±0.64 | 39.67±5.01 | 14.39±2.97 | 12.27±0.83 | 27.93±6.03 | 3.72:1±0.53 |
12M-wild-type (n=5) | 7.00±1.10 | 42.07±3.18 | 16.20±1.12 | 10.06±2.55 | 24.67±4.16 | 4.61±0.64 |
15M-wild-type (n=6) | 7.11±1.88 | 40.95±2.33 | 15.95±2.04 | 11.28±1.04 | 25.44±4.06 | 4.30±0.47 |
5M-SOX4 (n=12)# | 7.64±2.56 | 37.53±5.22 | 13.33±1.65* | 11.95±3.02 | 29.08±6.40 | 4.10±1.59 |
9M-SOX4 (n=12)# | 14.86±7.07** | 50.53±10.33* | 9.69±1.65** | 7.22±3.46* | 17.78±5.19* | 11.62±7.25** |
12M-SOX4 (n=11)# | 16.24±7.25** | 58.33±7.14** | 6.09±3.61** | 6.21±2.16* | 13.12±4.38** | 13.55±5.45** |
15M-SOX4 (n=9)# | 14.19±5.16** | 50.74±9.15* | 9.59±1.38*** | 7.15±3.29* | 16.74±4.42** | 10.48±4.08** |
Abbreviations: M, month; M/E ratio, myeloid-to-immature erythroid ratio.
Significant differences between the SOX4 transgenic fish and wild-type are indicated (*P<0.05; **P<0.01; ***P<0.001) by Mann–Whitney U-test.
Significant difference of groups between the SOX4 transgenic fish and wild-type are indicated (#P<0.001) by one-way analysis of variance (ANOVA).
A total number of 300 cells per zebrafish was counted to classify the distribution and subtypes of these hematopoietic cells.