Figure 1.

MiRs-143 and -145 modulate cell–cell contacts and migration of S3C cells. (a,b) S3C and WT cells were analyzed by western blot (a) or qRT-PCR (mean±S.E.M. of expression values relative to WT cells, n=3) (b). (c-e) sp-miR-143, sp-miR-145 or empty vector control S3C cells were analyzed by immunofluorescence or GFP detection (scale bar=30 μm) (c), western blot (d) and Transwell assay (mean±S.E.M. of the area covered by migrated cells, relative to the empty vector control, n=3) (e). Numbers below the lanes indicate signal intensity, normalized for loading and relative to the control sample. P-values: **P<0.01; ***P<0.001