Figure 6.

The inhibitory effect of CBX8 on cell migration and invasion depends on the down-regulation of Snail. (A, B) Both the cell migration and invasion assays were performed in the indicated stable cell lines as described in the Materials and methods. Representative images (100 ×) are shown. The columns, mean of three independent experiments; The bars indicate the SD. ** p < 0.01 and *** p < 0.001 using Student's t-test. (C-E) An in vivo lung metastasis model was established in nude mice using TE-1 cells stably expressing NC, shControl, shCBX8, shSnail or both, as indicated, as described in Materials and Methods. (C) Representative images of gross and H&E staining (Scale bars = 50 μm) of metastatic lung nodules. Numbers (D) and wet weights (E) of metastatic nodules per lung in the nude mice were summarized (n = 5). The bars indicate the SD. A two-tailed Student's t-test was used for statistical analysis.