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. 2017 Aug 17;7(14):3504–3516. doi: 10.7150/thno.19017

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Generation of a synthetic lymphoid tissue-like organoid in mice. (A) Fluorescence imaging of transplant tissues. Red, PNAd (one of the HEV-specific adhesion molecules) and green, B220. (B) Immunohistochemical staining of spleen and transplant tissues. Red, PECAM-1 (adhesion molecule expressed on endothelial cells of blood vessel) and bluish purple, B220. (C) Schematic of the procedure to study formation of antigen-specific IgG1 antibody under various conditions. (D) Specific IgG1 antibody was detected by ELISA in sera from SCID mice after various treatment as indicated. Reprinted with permission from ref. 131. Copyright 2004 Nature Publishing Group.