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. 2017 Aug 22;7(14):3595–3607. doi: 10.7150/thno.18974

Figure 1.

Figure 1

BM-MSCs augment migration, invasion and stemness of TNBC cells. (A) Representation of BT-549 or MDA-MB-231 cells co-cultured with BM-MSCs using Boyden chambers. (B) Migration of BT-549 and MDA-MB-231 cells was analyzed by Boyden chamber containing inserts of polycarbonate membranes with 8 μm pores. TNBC cells were seeded into upper chamber and exposed to BM-MSCs grown in medium supplemented with 1% FBS or medium containing 1% FBS as a chemoattractant (lower chamber) for 24 hours. (C) Invasion of BT-549 and MDA-MB-231 cells was performed at 72 hours as described above with membranes of trans-well coated with Matrigel. Representative photographs of at least three different experiments are shown. The results are expressed as percent of migrated/invaded cells and cells vs 1% FBS are reported as 100%. (D) For stemness markers analysis BT-549 and MDA-MB-231 cells were co-cultured with BM-MSCs for 5 days using a Boyden chamber containing inserts of polycarbonate membranes with 3 μm pores. Nanog and Sox2 mRNA levels were analyzed by Real Time-PCR. All experiments were performed at least three times. *P < 0.01 **P < 0.001 compared to control (medium with 1% FBS).