Fig. 4.

Inactive GDP-bound Rab5 reduced the level of FC and CE. a, b, c and d U937-derived macrophages were transfected for 72 h with plasmids encoding GFP-tagged inactive or active mutated forms of Rab5 as indicated. a Cells were incubated for 3 h with 80 μg/ml oxLDL and stained with filipin. Scale bars = 10 μm. b Cells were treated for 24 h with 80 μg/ml oxLDL and stained with nile red. Scale bars = 10 μm. b and d Fluorescence intensity of filipin and nile red was quantified following oxLDL incubation (n = 50, *P < 0.05; ***P < 0.001)