Fig. 5.

Inactive GDP-bound Rab5 impaired oxLDL internalization and disrupted the interaction between oxLDL and early endosomes in U937-derived macrophages. a, b and c U937-derived macrophages were transfected for 72 h with plasmids encoding GFP-Rab5-S34N and GFP-Rab5-Q79L as indicated. a Cells were loaded for 3 h with 40 μg/ml Dil-oxLDL before fixation. Scale bars = 10 μm. b Quantification of intracellular Dil-oxLDL was assayed after the incubation of Dil-oxLDL (n = 50, *P < 0.05). c Colocalization of internalized Dil-oxLDL and EEA-1 in U937-derived macrophages transfected with GFP-Rab5-S34N and GFP-Rab5-Q79L. Scale bars = 10 μm. The means ± SD of the PCC of 20–30 cells are shown