Figure 3.

Five-Repeat Micro-Dystrophin Ameliorates Dystrophic Pathology in Skeletal Muscle of DBA/2J-mdx Mice

(A) Representative photomicrographs of H&E (HE), Masson trichrome (MTC), and alizarin red staining. The blue color in MTC staining indicates fibrosis. The dark red color in alizarin red staining marks calcification. (B) Representative photomicrographs of macrophage and neutrophil immunohistochemical staining from the same serial sections shown in (A). Arrows mark inflammatory cells. Bar graphs show macrophage and neutrophil quantification. Error bars are mean ± SEM. (C) Myofiber size distribution in the quadriceps, tibialis anterior muscle (TA), and extensor digitorum longus muscle (EDL). (D) Quantification of the proportion of centrally nucleated myofibers. Error bars are mean ± SEM. Asterisks in photomicrographs indicate the same myofiber in serial sections. Asterisks in bar graphs indicate significantly different from other groups.