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. 2017 Jul 27;6:216–230. doi: 10.1016/j.omtm.2017.06.006

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© 2017 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Abnormal Heart Histology in DBA/2J Mice Reveals the Limitation of DBA/2J-mdx Mice as a Model for Studying DMD Heart Disease

(A) Representative full-view photomicrographs of H&E (HE), Masson trichrome (MTC), and alizarin red staining and dystrophin R17 immunostaining of the heart of DBA/2J, untreated, and AAV-treated DBA/2J-mdx mice. Selected areas of interest are numbered with 1, 2, and 3 to represent the right ventricular (RV) wall, septum, and myocardium, respectively. (B) A close view of H&E-stained images of boxed areas 1, 2, and 3 in (A). (C) A close view of Masson trichrome-stained images of boxed areas 1, 2, and 3 in (A). (D) A close view of alizarin red-stained images of boxed areas 1, 2, and 3 in (A).