Skip to main content
. Author manuscript; available in PMC: 2017 Dec 12.
Published in final edited form as: Nat Med. 2017 Jun 12;23(7):829–838. doi: 10.1038/nm.4346

Figure 1.

Figure 1

Thermoneutral housing relieves stress and augments inflammation. (a–f) 6 week-old WT C57BL/6 male mice were housed at either standard (TS; 22°C) or thermoneutral (TN; 30°C) conditions for a minimum of 3 weeks. (a) Expression of the indicated genes in brown adipose tissue (n = 5 per group). (b) Serum corticosterone levels (n = 7 per group). (c) Expression of the indicated genes in the spleen (n = 3 per group). (d) Upregulated gene expression pathways in PBMCs and genes within pathways, determined by RNASeq analysis (n = 2 per group). (e) Serum TNF and IL-6 level fold change of unstimulated. TN-housed (n = 29 per group) mice; LPS-stimulated, TS housed (n = 4 per group); and TN-housed (n = 3 per group) mice compared to unstimulated, TS-housed (n = 31 per group) mice. (f) Fold change in splenocyte or BMDC supernatants derived from TS- or TN-housed mice for TNF (n = 3 per group) and IL-6 (n = 5 per group). (g) Serum fold change for TNF in LPS-treated, TN-housed (n = 3 per group) and TN-housed mice also treated with 100mg/L corticosterone in drinking water (n = 4 per group) compared to LPS-treated, TS-housed mice (n = 4 per group). For bar graphs, data represents mean + s.e.m. (a–c and g) A single experiment. (e) Samples represent combined data of 4 individual experiments. (f) A representative of 2 individual experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. (a–c and e–f) Student’s t-test. (g) One way ANOVA with post hoc Tukey’s test.