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. 2017 Aug 3;8(8):e2966. doi: 10.1038/cddis.2017.350

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Effect of DEHP on γH2AX pattern. (a) Immunolabeling of the oocyte chromosomes with anti-SYCP3 (red) and anti-γH2AX (green) antibodies. (b) Percentage of positive γH2AX oocytes (79.36±3.97% and 81.40±0.62%, respectively), were significantly higher than that of control group (54.70±0.61% P<0.01). (c) Percentage of normal, negative or weak γH2AX staining and defective (strong γH2AX staining) oocytes after six days of culture with or without DEHP. (d) Percentage of oocytes at the pachytene and diplotene stages showing negative, weakly and strongly positive γH2AX staining after six days of culture with or without DEHP. (*P<0.05; **P<0.01)