Figure 2.

IL-13-induced autophagy is dependent on IGFBP-3 expression. BEAS-2B cells were transfected with siRNA against IGFBP-3 or scrambled siRNA (SCR) for 72 h. (a) The expression of IGFBP-3 was assessed by western blotting and real-time PCR. Values represent the mean±S.E.M. (n=5), ***P<0.001 versus control. (b–k) BEAS-2B cells transfected with siIGFBP-3 or SCR were treated with or without 20 ng/ml IL-13 for 72 h. (b–e) Western blot analysis of Beclin-1, LC3 and P62 and densitometric quantification of bands. Values represent the mean±S.E.M. (n=3), ***P<0.001 versus normal control. (f and g) Real-time PCR analysis of miR-34 and miR-449 expression. Values represent the mean±S.E.M. (n=3), *P<0.05, **P<0.01, ***P<0.001 versus control. (h–j) Western blot analysis of Nur77 and Bcl-2 and densitometric quantification of bands. Values represent the mean±S.E.M. (n=3), ***P<0.001 versus control. (k) Co-immunoprecipitation analysis of the interaction between Beclin-1, Bcl-2 and Nur77