Figure 6.

IGFBP-3 is a target of miR-34/449. (a) Sequence alignment between miR-34/449 and the 3′-UTR of human IGFBP-3. Complementary bases between the sequences are shown in red. The sequence of the mutant MALAT1 construct is also shown. (b) Dual-luciferase reporter assay of BEAS-2B cells co-transfected with IGFBP-3 3′-UTR-WT or IGFBP-3 3′-UTR-Mut, and miR-34 mimic or miR-NC. Data are presented as the mean±S.D. from six separate experiments. ***P<0.01. (c) Dual-luciferase reporter assay of BEAS-2B cells co-transfected with IGFBP-3 3′-UTR-WT or IGFBP-3 3′-UTR-Mut and miR-449 mimic or miR-NC. Data are presented as the mean±S.D. from six separate experiments. ***P<0.01