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. 2017 Aug 24;8(8):e3008. doi: 10.1038/cddis.2017.362

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Adropin-deficiency results in loss of p-eNOS and T_reg cells. (a) Confocal immunofluorescence analysis showing diminutive areas of colocalized DNA in blue, adropin in green and VEGFR2 in red, the overlap of adropin and VEGFR2 (yellow staining in the merged image). (b) Confocal immunofluorescence analysis showing small areas of co-localized DNA in blue, CD31 in red and p-eNOS in green, overlap of CD31 and p-eNOS (yellow staining in the merged image) in the endothelial layers. (c) Colocalization of DNA (blue), CD4 (red) and Foxp3 (green) indicates T_reg cells formation in myocardial. (d) Colocalization of DNA (blue), CD4 (red) and Foxp3 (green) indicates T_reg cells formation in pancreatic tissues. Original magnification: × 400