Figure 3.

Effect of rKlotho administration on Tac-induced mitochondrial ultrastructure and MnSOD expression in an experimental mouse model. Representative transmission electron microscopy images of the mitochondrial ultrastructure in proximal tubules (a–d) and its quantification in a mouse kidney (i–k). MnSOD immunofluorescence (green fluorescence) (e–h) and its quantification (l) in each group. The Tac groups showed mitochondrial disorders such as a lower number, area, and size of mitochondria, and rKlotho administration reversed those changes. Note that the reduction and intensity of MnSOD-positive dots was also improved by rKlotho administration. Scale bars=1 μm (a–d) or 10 μm (e–h). The data are presented as the mean±S.E. ^#P<0.05 versus the VH group; ^$P<0.05 versus the rKlotho groups; ^@P<0.05 versus the Tac group