Figure 8.

Klotho improves the mitochondrial membrane potential in Tac-treated HK-2 cells. HK-2 cells were seeded in culture plates at 90% confluence. On the next day, the cells were treated with Tac (60 μg/ml) in the absence or presence of 1 μg/ml rKlotho and 25 μM LY294002 (LY, PI3K inhibitor) for 12 h. The cells were labeled with JC-1 to evaluate the mitochondrial membrane potential (ΔΨm) and then analyzed by flow cytometry and confocal microscopy. (a and b) Flow cytometry plots and a quantitative graph for JC-1 labeling. (c) Confocal microscopy images for JC-1 staining. LY; LY294002. Scale bar=20 μm. The data are presented as the mean±S.E. ^#P<0.05 versus the VH group; ^$P<0.05 versus the rKlotho groups; ^@P<0.05 versus the Tac group; ^!P<0.05 versus the Tac+rKlotho group