Figure 5.

Induced ERS in SMMC-7721 cells. (a) Western blot and densitometric analyses for hTERT, p65, and GRP-78 after SMMC-7721 cells were treated with the title compound (20, 40, and 80 nm) for 24 h. At the indicated time points, cells were harvested for qRT-PCR analysis of GRP-78 mRNA expression. β-actin was used as the internal control. (b) Immunofluorescence assay for the expression of GRP-78 after SMMC-7721 cells treated with the title compound (20, 40, and 80 nm) for 24 h and immunostained with anti-GRP-78 antibodies. Fluorescence-labeled cells were analyzed under a fluorescence microscope. (c) Western blot and densitometric analyses for hTERT, IκBα, p65, and GRP-78 treated with the title compound (40 nm) for 0, 3, 6, 12, 24, and 48 h. β-actin was used as the internal control. For the statistics of each panel in this figure, data are expressed as mean±S.D. (n=3); scale bars are 25 μm; *P<0.05 versus control, **P<0.01 versus control, ***P<0.001 versus control