Figure 5.

CDDO-Im pretreatment inhibit oxidative stress and protect against mitochondrial dysfunction during hepatic I/R injury. (a) Serum levels of mtDNA in mice at 6 h after reperfusion. (b) Effects of the CDDO-Im and DMSO on the levels of MDA in mice livers at 6 h after reperfusion. (c,d) Representative pictures of mitochondrial membrane potential of primary hepatocytes isolated from WT, which subjected to H/R injury in the CDDO-Im and DMSO pretreatment group (original magnification, × 400). The ratio of green to red fluorescence intensity were determined. (e,f) Representative pictures of mitochondrial ROS accumulation of primary hepatocytes after H/R injury in both groups (original magnification, × 200). The mitochondrial ROS was expressed by relative red area of the total picture. Values expressed as mean±S.E.M. (n=4-6). *P<0.05 and **P<0.01