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. 2017 Aug 10;8(8):e2988. doi: 10.1038/cddis.2017.387

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Both LC3 and Ulk1 interact with PARP-1. (a) 786-O cells were treated with or without ST for 3 h, cells were lysed, and precipitated using the indicated antibodies. The immunoprecipitates were resolved by electrophoresis and probed by immunoblotting with the indicated antibodies. (b) Immunoprecipitation was performed for the lysate extracted from 786-O cells using either the antibody of LC3 or pUlk1. IgG: the negative control antibody. (c and d) After treated with or without fresh medium for 2 h, immunoprecipitation was performed for the Cyto and Nu fractions extracted from HEK293T cells using the antibody of LC3 and pUlk1, respectively. IgG (L): the light chain of IgG. The immunoprecipitates were resolved by electrophoresis and probed by immunoblotting with the indicated antibodies. At least three independent experiments were performed